And here are the results of the blender comparison. My interest was in pushing the use of the beta-glucosidase found in the Japanese Indigo species to affect other varieties of indigo, in this case, Indigofera Suffruticosa. A paper titled
rβ-Glucosidase in the Indigo Plant: Intracellular Localization and Tissue Specific Expression in Leaves
compiled by a team of Japanese scholars goes thru some of the details of cell structure. This paper is located on https://academic.oup.com. I am not a scientist but am using the culture of grandmothers’ knowledge to try this. One day I will hook up with a scientist to understand WHY this works.
Let’s play “What was in the fresh leaf blender?” Please note these skeins have not been washed! More to come after washing to see what really sticks. ANSWERS BELOW!
1. Which skein is blender indigofera suffruticosa?
2. Which skein is blender Japanese Indigo?
3. Which skein is a “blend” of both Japanese Indigo and Indigofera Suffruticosa?
4. Which is the leftover skein for all season?
Please note the @botanicalcolors hoops in use!
debmcclintock 
When I did a fresh leaf blender japanese indigo, it came out like your 2nd skein from left. It was lighter after rinsing. Then I reused the dye and it looked like your 3rd skein. I’m guessing the suffruticosa is the first skein.
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Guess Again! I’ll post the answer after I wash everything this week.
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Once more, and I stop. First skein is the blend. 2nd is the japanese. 3rd is the leftover. 4th is suffruticosa.
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Not yet!
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I don’t know but you sure are having fun!
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